Handball around the world is more dynamic and faster than ever before. The modification of rules and implementing various training methodologies resulted in drastic change in the playing format of the game. Handball is a strenuous contact Olympic team sport that places emphasis on speed, power, agility, strength, flexibility and endurance arm throwing, hitting, blocking, and pushing. Thirty two (32) male handball players were selected from Department of Physical Education and Sports Sciences, Annamalai University, Chidambram, Tamilnadu, India. These players were classified into four groups as backs (n = 12), wings (n = 7), pivots (n = 7) and goalkeepers (n = 6) respectively. The collected data was analysed using one way Analysis of variance (ANOVA). When F ratio was found significant, Scheffe’s post hoc test was applied to know the difference between the four groups. It is concluded that handball players of different playing position showed significant difference on agility. The coaches and physical education teachers use this information while supplementing training to players and also use this information in the process of designing training program.