The present paper aimed at measuring the Academic Achievement of Education subject of High secondary level students in Nadia district at Kalyani block. A survey of 62 sample from rural and urban (rural-30 & urban- 32) were conducted. One principle variable- ‘Academic Achievement of Education subject’ was considered. Researcher collected academic score of Education subject from the XI and XII classes. Mean and SD as Descriptive Statistics, and‘t’ test as Inferential Statistics were employed to find the significance of difference among the gender towards the Academic Achievement of Education subject’. Finally found the significant difference from four null hypotheses. And also found that the Academic Achievement (Education subject) of urban students were significantly higher than rural students.